Premium
LC‐MS determination and pharmacokinetic studies of paeonol in rat plasma after administration of different compatibility of Su‐Xiao‐Xin‐Tong prescriptions
Author(s) -
Xiao Yao,
Zhang Yinghao,
Sheng Yuxin,
Zhang Jinlan
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.964
Subject(s) - chemistry , paeonol , chromatography , formic acid , electrospray ionization , pharmacokinetics , electrospray , mass spectrometry , detection limit , quadrupole mass analyzer , high performance liquid chromatography , pharmacology , medicine , alternative medicine , pathology
A rapid and specific liquid chromatographic/electrospray ionization mass spectrometric (LC/ESI–MS) method has been developed and validated for the identification and quantification of paeonol in rat plasma. Paeonol and internal standard were isolated from plasma samples by liquid–liquid extraction with chloroform. The chromatographic separation was accomplished on a Zorbax‐SB C 18 column (100 × 2.1 mm, 3.5 µm). The mobile phase consisted of acetonitrile and 0.1% aqueous formic acid (64:36) was delivered at a flow rate of 0.2 mL/min. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring mode via electrospray ionization source. Linearity was established for the range of concentrations 0.0525–15.8 µg/mL with a coefficient correlation ( r ) of 0.9995. The intra‐ and inter‐day precision (RSD%) was lower than 9.34% and accuracy ranged from 93.7 to 102.3%. The lower limit of quantification was 0.0525 µg/mL. The proposed method was used to determine the concentration of paeonol for pharmacokinetic studies. The pharmacokinetics of different compatibility prescriptions of Su‐Xiao‐Xin‐Tong were studied and compared. Copyright © 2008 John Wiley & Sons, Ltd.