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Resolution of enantiomers of DL ‐amino acids on silica gel plates impregnated with optically pure (—)‐quinine
Author(s) -
Bhushan R.,
Arora Meenakshi
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.96
Subject(s) - chemistry , enantiomer , silica gel , chloroform , chromatography , resolution (logic) , reagent , alanine , amino acid , acetic acid , valine , solvent , ethyl acetate , methionine , organic chemistry , biochemistry , artificial intelligence , computer science
TLC resolution of enantiomers from racemic amino acids was achieved on silica gel plates impregnated with optically pure (—)‐quinine. The successful solvent systems were butanol–chloroform–acetic acid (3:7:5, v/v) for DL ‐methionine; 6:8:4, v/v for alanine; 10:1:4; v/v for threonine; and ethyl acetate–carbon tetrachloride–propionic acid (10.5:6.5:3.5, v/v) for valine. Minimum detection limits were found to be different for each of the amino acid, ranging between 0.9 and 3.7 µg. The effects of concentration of impregnating reagent, temperature and pH on resolution of enantiomers have been studied in details. Copyright © 2001 John Wiley & Sons, Ltd.