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Novel molecularly imprinted monolithic column for selective on‐line extraction of ciprofloxacin from human urine
Author(s) -
Yan Hongyuan,
Row Kyung Ho
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.957
Subject(s) - chemistry , chromatography , monolithic hplc column , ciprofloxacin , molecularly imprinted polymer , extraction (chemistry) , detection limit , solvent , aqueous solution , solid phase extraction , matrix (chemical analysis) , selectivity , high performance liquid chromatography , biochemistry , organic chemistry , catalysis , antibiotics
Water‐compatible ciprofloxacin‐imprinted monolithic columns were synthesized in water‐containing systems for selective extraction of ciprofloxacin from human urine samples. Methanol–water (10:3, v/v) was used as a porogenic solvent and the obtained monolithic imprinted polymers reveal high selectivity to ciprofloxacin in an aqueous environment; the affinity can be easily controlled by adjusting the pH of the mobile phase. Owing to the unique porous structure and flow‐through channels existing in the network skeleton of the monolithic MIP, urine samples could be directly injected into the column, proteins and other biological matrix were quickly washed out and ciprofloxacin was selectively retained and enriched. Good linearity was obtained from 0.08 to 400 mg/L ( r = 0.998) with the relative standard deviations less than 3.6%. The limit of detection of the method was 0.04 mg/L and the recoveries were more than 94.5% at three different concentrations. Moreover, by increasing the injection volume to 2.0 mL, the sensitivity of the method could be improved 100‐fold according to the peak height of ciprofloxacin. This expedient greatly simplified the overall procedure, resulting in a rapid and efficient sample analysis while maintaining precision and accuracy. Copyright © 2007 John Wiley & Sons, Ltd.