Premium
Quantitative determination of domperidone in human plasma by ultraperformance liquid chromatography with electrospray ionization tandem mass spectrometry
Author(s) -
Xu DongHang,
Lou HongGang,
Yuan Hong,
Jiang Bo,
Zhou Quan,
Zhang ZhongMiao,
Ruan ZouRong
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.952
Subject(s) - chemistry , chromatography , domperidone , protein precipitation , analyte , formic acid , selected reaction monitoring , electrospray ionization , bioanalysis , liquid chromatography–mass spectrometry , tandem mass spectrometry , detection limit , mass spectrometry , analytical chemistry (journal) , neuroscience , dopamine , biology
A simple and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for determining domperidone in human plasma. The analyte and internal standard (IS; mosapride) were isolated from plasma samples by protein precipitation with methanol (containing 0.1% formic acid). The chromatographic separation was performed on an Xterra MS C 18 Column (2.1 × 150 mm, 5.0 µm) with a gradient programme mobile phase consisting of 0.1% formic acid and acetonitrile at a flow rate of 0.30 mL/min. The total run time was 4.0 min. The analyses were carried out by multiple reaction monitoring using the parent‐to‐daughter combinations m/z 426 → 175 and m/z 422 → 198 (IS). The areas of peaks from the analyte and IS were used for quantification of domperidone. The method was validated according to the FDA guidelines on bioanalytical method validation. Validation results indicated that the lower limit of quantification was 0.2 ng/mL, and the assay exhibited a linear range of 0.2–60.0 ng/mL and gave a correlation coefficient ( r 2 ) of 0.999 or better. Quality control samples (0.4, 0.8, 15 and 50 ng/mL) in six replicates from three different analytical runs demonstrated an intra‐assay precision (RSD) 4.43–6.26%, an inter‐assay precision 5.25–7.45% and an overall accuracy (relative error) of <6.92%. The method can be applied to pharmacokinetic and bioequivalence studies of domperidone. Copyright © 2007 John Wiley & Sons, Ltd.