Premium
HPLC assay for determination of amphotericin B in biological samples
Author(s) -
Espada R.,
Josa J. M.,
Valdespina S.,
Dea M. A.,
Ballesteros M. P.,
Alunda J. M.,
Torrado J. J.
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.947
Subject(s) - chromatography , chemistry , amphotericin b , high performance liquid chromatography , pharmacokinetics , chromatographic separation , acetonitrile , pharmacology , antifungal , medicine , dermatology
Abstract A fast and selective HPLC method for assaying amphotericin B in biological samples was developed and validated. The chromatographic separation was achieved in less than 12 min on a reverse‐phase C 18 column using an acetonitrile–acetic acid–water (52:4.3:43.7, v/v/v) mixture as mobile phase. The flow rate was 1 mL/min and the effluent was monitored at 406 nm. A linear response over the concentration range 0.1–10.0 µg/mL was obtained. Intra‐day and inter‐day RSDs were below 5% for all the sample types. This new HPLC method was applied to assay amphotericin B in plasma and several tissue samples such as kidney, liver, spleen and bone marrow. Application of this method to pharmacokinetic studies in mice and dog is provided. Copyright © 2007 John Wiley & Sons, Ltd.