HPLC assay for determination of amphotericin B in biological samples | Zendy

Espada R. | Zendy; Josa J. M. | Zendy; Valdespina S. | Zendy; Dea M. A. | Zendy; Ballesteros M. P. | Zendy; Alunda J. M. | Zendy; Torrado J. J. | Zendy

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HPLC assay for determination of amphotericin B in biological samples

Author(s) -

Espada R.,

Josa J. M.,

Valdespina S.,

Dea M. A.,

Ballesteros M. P.,

Alunda J. M.,

Torrado J. J.

Publication year - 2008

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.947

Subject(s) - chromatography , chemistry , amphotericin b , high performance liquid chromatography , pharmacokinetics , chromatographic separation , acetonitrile , pharmacology , antifungal , medicine , dermatology

A fast and selective HPLC method for assaying amphotericin B in biological samples was developed and validated. The chromatographic separation was achieved in less than 12 min on a reverse‐phase C 18 column using an acetonitrile–acetic acid–water (52:4.3:43.7, v/v/v) mixture as mobile phase. The flow rate was 1 mL/min and the effluent was monitored at 406 nm. A linear response over the concentration range 0.1–10.0 µg/mL was obtained. Intra‐day and inter‐day RSDs were below 5% for all the sample types. This new HPLC method was applied to assay amphotericin B in plasma and several tissue samples such as kidney, liver, spleen and bone marrow. Application of this method to pharmacokinetic studies in mice and dog is provided. Copyright © 2007 John Wiley & Sons, Ltd.

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