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A simple and cost effective method for the quantification of 8‐hydroxy‐2′‐deoxyguanosine from urine using liquid chromatography tandem mass spectrometry
Author(s) -
Crow Brian,
Bishop Michael,
Kovalcik Kasey,
Norton Dean,
George Joe,
Bralley J. Alexander
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.946
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , electrospray ionization , liquid chromatography–mass spectrometry , deoxyguanosine , sample preparation , 8 hydroxy 2' deoxyguanosine , selected reaction monitoring , oxidative stress , dna oxidation , biochemistry , oxidative damage
A rapid and high‐throughput method for the determination of urinary levels of the oxidative stress biomarker, 8‐hydroxy‐2′‐deoxyguanosine (8‐OH‐dG), has been developed and validated using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC‐MS/MS). The assay features a cheap and readily available non‐isotopic internal standard, a single‐step filtration sample preparation, and a total analysis time of 6 min including column re‐equilibration. The method was validated based on linearity, accuracy (100–106%), precision (CV < 7%), sample preparation stability (≤5%, 72 h). Intra‐laboratory patient ranges were established comparing children and adults ( n = 345). Copyright © 2007 John Wiley & Sons, Ltd.