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Development and validation of a method for the analysis of cafenstrole and its metabolite in brown rice grains and rice straw using high‐performance liquid chromatography
Author(s) -
Abd ElAty A. M.,
Lee GoWoon,
Mamun M. I. R.,
Choi JeongHeui,
Cho SoonKil,
Shin HoChul,
Shim JaeHan
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.934
Subject(s) - metabolite , chemistry , chromatography , acetic acid , extraction (chemistry) , detection limit , high performance liquid chromatography , straw , solid phase extraction , rice straw , biochemistry , inorganic chemistry
The present work reports the extraction and clean‐up procedures, as well as the chromatographic conditions developed, for the determination of cafenstrole and its metabolite (CHM‐03) residues in brown rice grains and rice straw using HPLC‐UV detection. The method makes use of an Apollo C 18 column and acetonitrile : water : acetic acid as a mobile phase for both cafenstrole and its metabolite in rice and rice straw. Using these conditions cafenstrole and its metabolite were resolved with a retention time ( R t ) of less than 14 min. The analytes were confirmed using positive atmospheric pressure ionization LC‐MS with selected ion monitoring. The average recoveries of cafenstrole were found to be 87.0–92.5 and 87.6–88.3%. However, they ranged from 81.5 to 81.6% and from 76.1 to 78.5% for cafenstrole metabolite (CHM‐03), in rice grains and rice straw, respectively, with relative standard deviations ranging from 1.4 to 6.6%. The limits of detection (LODs) of both cafenstrole and its metabolite were 0.002 and 0.02 ppm and 0.025 and 0.04 ppm, respectively. Field trials with recommended or double the recommended dose revealed that the herbicide could safely be recommended for application in rice and rice straw as no residues were detected in the harvest samples. Copyright © 2007 John Wiley & Sons, Ltd.