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Sensitive liquid chromatography tandem mass spectrometry method for the quantification of sitagliptin, a DPP‐4 inhibitor, in human plasma using liquid–liquid extraction
Author(s) -
Nirogi Ramakrishna,
Kandikere Vishwottam,
Mudigonda Koteshwara,
Komarneni Prashanth,
Aleti Raghupathi,
Boggavarapu Rajeshkumar
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.926
Subject(s) - chromatography , chemistry , sitagliptin , sitagliptin phosphate , liquid chromatography–mass spectrometry , analyte , extraction (chemistry) , selected reaction monitoring , mass spectrometry , electrospray , detection limit , tandem mass spectrometry , liquid–liquid extraction , calibration curve , metformin , diabetes mellitus , endocrinology , medicine
A sensitive high‐performance liquid chromatography–positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of sitagliptin, a DPP‐4 inhibitor, in human plasma. Following liquid–liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse‐phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H] + ions, m/z 408–235 for sitagliptin and m/z 310–148 for the internal standard. The assay exhibited a linear dynamic range of 0.1–250 ng/mL for sitagliptin in human plasma. The lower limit of quantification was 0.1 ng/mL with a relative standard deviation of less than 6%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 2.0 min for each sample made it possible to analyze more than 300 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic studies. Copyright © 2007 John Wiley & Sons, Ltd.