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Stereoselective determination of demethyl‐ and didemethyl‐citalopram in rat plasma and brain tissue by liquid chromatography with fluorescence detection using precolumn derivatization
Author(s) -
Millán S.,
Goicolea M. A.,
Sánchez A.,
GómezCaballero A.,
Sampedro M. C.,
Unceta N.,
Barrio R. J.
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.922
Subject(s) - chemistry , derivatization , chromatography , fluorescence , stereoselectivity , citalopram , high performance liquid chromatography , brain tissue , biochemistry , serotonin , physics , quantum mechanics , medicine , receptor , anatomy , catalysis
A rapid and sensitive HPLC enantioselective method with fluorescence detection was developed to determine (−)‐( R ) and (+)‐( S ) enantiomers of the metabolites of citalopram, demethyl‐ and didemethyl‐citalopram in plasma and brain tissue. This assay involves pre‐column chiral derivatization with (−)‐( R )‐1‐(1‐naphthyl)ethyl isocyanate followed by separation on a normal‐phase silica column. The developed liquid–liquid extraction procedure permits quantitative determination of analytes with recoveries ranged between 81 and 88% with intra‐ and inter‐day relative standard deviations less than 10.5%. Linearity was obtained over the concentration range 5–1000 ng/mL and 100–10,000 ng/g for spiked drug‐free plasma and brain tissue, respectively, with detection limits lower than 2.1 ng/mL and 42.8 ng/g. Copyright © 2007 John Wiley & Sons, Ltd.