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Simultaneous determination of swertiamarin and its metabolites (5 Z )‐5‐ethylidene‐8‐hydroxy‐3,4,5,6,7,8‐hexahydro‐1 H ‐pyrano[3,4‐ c ]pyridin‐1‐one and erythrocentaurin in broth of Aspergillus niger by HPLC
Author(s) -
Jun Chang,
XueMing Zhao,
ChangXiao Liu,
TieJun Zhang
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.913
Subject(s) - chemistry , chromatography , organic chemistry
When cultivated with Aspergillus niger , swertiamarin, an important drug, is rapidly transformed into erythrocentaurin and (5 Z )‐5‐ethylidene‐8‐hydroxy‐3,4,5,6,7,8‐hexahydro‐1 H ‐pyrano[3,4‐ c ]pyridin‐1‐one (M 1 ), a new compound with high anti‐inflammatory activity. A simple and rapid HPLC method for simultaneous determination of swertiamarin and its two metabolites in broth of A. niger is described. The chromatographic separation was achieved on a C 18 ODS column (250 × 4.6 mm i.d.) by gradient elution with 0.04% formic acid in water and 0.04% formic acid in acetonitrile as the gradient mixtures. The flow rate was 1 mL/min, the detection wavelength was 237 nm and the column temperature was kept at 30°C. The retention times of swertiamarin, erythrocentaurin and M 1 were 14.6, 16.8 and 24.8 min, respectively. The mean absolute recoveries of three analysts were over 96%. Quantification limits were 0.02 µg/mL for swertiamarin and 0.05 µg/mL for both of the two metabolites. The method was applied for the quantification of swertiamarin and its two metabolites during the fermentation process and the evaluation of the bioavailabilities in the Caco‐2 monolayer. Copyright © 2007 John Wiley & Sons, Ltd.