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Round‐robin evaluation of a solid‐phase microextraction–gas chromatographic method for reliable determination of trace level ethylene oxide in sterilized medical devices
Author(s) -
Harper Thomas,
Cushinotto Lisa,
Blaszko Nancy,
Arinaga Julie,
Davis Frank,
Cummins Calvin,
DiCicco Michael
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.908
Subject(s) - solid phase microextraction , ethylene oxide , chromatography , chemistry , detection limit , gas chromatography , trace (psycholinguistics) , trace amounts , gas chromatography–mass spectrometry , mass spectrometry , organic chemistry , medicine , linguistics , philosophy , alternative medicine , pathology , copolymer , polymer
Medical devices that are sterilized with ethylene oxide (EtO) retain small quantities of EtO residuals, which may cause negative systemic and local irritating effects, and must be accurately quantified to ensure non‐toxicity. The goal of this round‐robin study is to investigate the capability of a novel solid‐phase microextraction–gas chromatographic (SPME‐GC) method for trace‐level EtO residuals analysis: three independent laboratories conducted a guided experiment using this SPME‐GC method, in assessing method performance, ruggedness and the feasibility of SPME fibers. These were satisfactory across the independent laboratories, at the 0.05–5.00 ppm EtO range. This method was then successfully applied to analyze EtO residuals in several sterilized/aerated medical devices of various polymeric composition, reliably detecting and quantifying the trace levels of EtO residuals present (∼0.05 ppm EtO). SPME is a feasible alternative for quantifying trace‐level EtO residuals in sterilized medical devices, thereby lowering the limit of quantification (LOQ) by as much as two to three orders of magnitude over the current GC methodology of direct liquid injection. Copyright © 2007 John Wiley & Sons, Ltd.