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Synthesis of 4‐[2‐( N , N ‐dimethylamino)ethylaminosulfonyl]‐7‐ N ‐methylhydrazino‐2,1,3‐benzoxadiazole (DAABD‐MHz) as a derivatization reagent for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry
Author(s) -
Santa Tomofumi,
AlDirbashi Osama Y,
Ichibangase Tomoko,
Rashed Mohamed S,
Fukushima Takeshi,
Imai Kazuhiro
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.903
Subject(s) - chemistry , derivatization , reagent , chromatography , electrospray ionization , mass spectrometry , dissociation (chemistry) , tandem mass spectrometry , electrospray , organic chemistry
Benzofurazan derivatization reagent, 4‐[2‐( N , N ‐dimethylamino)ethylaminosulfonyl]‐7‐ N ‐methylhydrazino‐2,1,3‐benzoxadiazole (DAABD‐MHz), for aldehydes in liquid chromatography/electrospray ionization–tandem mass spectrometry (LC/ESI‐MS/MS), was synthesized. DAABD‐MHz reacted with aliphatic aldehydes under mild conditions. The generated derivatives were separated on a reversed‐phase column and detected by ESI‐MS/MS with detection limits of 30–60 fmol on‐column. Upon collision‐induced dissociation, a single and intense fragment ion at m / z 151 was observed. These results suggested that DAABD‐MHz was suitable as a derivatization reagent in LC/ESI‐MS/MS. Copyright © 2007 John Wiley & Sons, Ltd.