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Analysis of polyamines as carbamoyl derivatives in urine and serum by liquid chromatography–tandem mass spectrometry
Author(s) -
Byun Jeong Ah,
Lee Sang Hee,
Jung Byung Hwa,
Choi Man Ho,
Moon Myeong Hee,
Chung Bong Chul
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.898
Subject(s) - chemistry , chromatography , urine , putrescine , spermine , liquid chromatography–mass spectrometry , polyamine , mass spectrometry , tandem mass spectrometry , detection limit , selected reaction monitoring , biochemistry , enzyme
A quantitative analysis of polyamines in urine and serum by liquid chromatography–tandem mass spectrometry (LC‐MS/MS) is described. The polyamines were carbamylated with isobutyl chloroformate, extracted with diethyl ether under pH 9.0, and analyzed by LC‐MS/MS with single reaction monitoring mode. The limit of quantification was 1 ng/mL based on a signal‐to‐noise ratio >3, and the correlation coefficient ( r 2 ) for the calibration curves was >0.99 for both urine and serum samples. The present method was applied to urine and serum samples from 30 breast cancer patients and 30 normal female controls. There was no significant difference in the urinary polyamine levels between breast cancer patients and controls. However, 1,3‐diaminopropane, putrescine, spermine and N ‐acetylspermidine levels in serum increased in breast cancer patients. These four serum polyamines may be a good index to study both production and metabolism of polyamines, and a useful tool in assessment of the polyamine status of breast cancer patients. Copyright © 2007 John Wiley & Sons, Ltd.