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The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis
Author(s) -
Rammouz Georges,
Lacroix Marlène,
Garrigues Jean Christophe,
Poinsot Véréna,
Couderc François
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.893
Subject(s) - derivatization , chemistry , chromatography , capillary electrophoresis , amine gas treating , detection limit , naphthalene , fluorescence , high performance liquid chromatography , laser induced fluorescence , organic chemistry , physics , quantum mechanics
Abstract This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene‐2,3‐dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene‐2,3‐dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks. Copyright © 2007 John Wiley & Sons, Ltd.