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Rapid and simple liquid chromatography tandem mass spectrometry method for the quantification of zidovudine in rat plasma
Author(s) -
Mudigonda Koteshwara,
Jukanti Raju,
Apte Shashank S.,
Kishan Veerabrahma,
Maurya Santosh,
Kandikere Vishwottam,
Nirogi Ramakrishna
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.888
Subject(s) - chromatography , chemistry , zidovudine , electrospray ionization , analyte , mass spectrometry , detection limit , tandem mass spectrometry , standard curve , selected reaction monitoring , pharmacokinetics , solid phase extraction , liquid chromatography–mass spectrometry , sample preparation , extraction (chemistry) , electrospray , human immunodeficiency virus (hiv) , medicine , family medicine , viral disease
Abstract A high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry method was developed and validated for the quantification of zidovudine in rat plasma. Following solid‐phase extraction, the analytes were separated using an isocratic mobile phase on a reverse phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective [M + H] + ions, m/z 268/127 for zidovudine and m/z 230/112 for the internal standard. The method exhibited a linear dynamic range of 5–500 ng/mL for zidovudine in rat plasma. The lower limit of quantification was 5 ng/mL with a relative standard deviation of less than 8%. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 1.5 min for each sample made it possible to analyze more than 400 plasma samples per day. The validated method was applied for pharmacokinetic studies of the novel drug delivery systems of zidovudine in rats. Copyright © 2007 John Wiley & Sons, Ltd.