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Determination of faropenem in human plasma and urine by liquid chromatography–tandem mass spectrometry
Author(s) -
Gao Shouhong,
Chen Wansheng,
Tao Xia,
Miao Haijun,
Yang Shaolin,
Wu Rong
Publication year - 2008
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.886
Subject(s) - chemistry , chromatography , protein precipitation , electrospray ionization , mass spectrometry , tandem mass spectrometry , selected reaction monitoring , liquid chromatography–mass spectrometry , formic acid , analyte , sample preparation , electrospray , pharmacokinetics , detection limit , urine , biochemistry , pharmacology , medicine
A simple, rapid and sensitive liquid chromatography/electrospray tandem mass spectrometry (LC‐MS/MS) quantitative detection method, using cefalexin as internal standard, was developed for the analysis of faropenem in human plasma and urine. After precipitation of the plasma proteins with acetonitrile, the analytes were separated on a C 18 reversed‐phase column with 0.1% formic acid–methanol (45:55, v/v) and detected by electrospray ionization mass spectrometry in positive multiple reaction monitoring mode. Calibration curves with good linearities ( r = 0.9991 for plasma sample and r = 0.9993 for urine sample) were obtained in the range 5–4000 ng/mL for faropenem. The limit of detection was 5 ng/mL. Recoveries were around 90% for the extraction from human plasma, and good precision and accuracy were achieved. This method is feasible for the evaluation of pharmacokinetic profiles of faropenem in humans, and to our knowledge, it is the first time the pharmacokinetic of faropenem has been elucidated in vivo using LC‐MS/MS. Copyright © 2007 John Wiley & Sons, Ltd.