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Sensitive liquid chromatography/mass spectrometry assay for the quantification of azithromycin in human plasma
Author(s) -
Liu Fei,
Xu Yu,
Huang Jinchang,
Gao Shu,
Guo Qingxiang
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.883
Subject(s) - chemistry , chromatography , protein precipitation , formic acid , electrospray ionization , ammonium acetate , methanol , mass spectrometry , calibration curve , liquid chromatography–mass spectrometry , high performance liquid chromatography , detection limit , organic chemistry
A simple, rapid, sensitive and specific liquid chromatography/electrospray ionization mass spectrometry method was developed and validated to quantify azithromycin in human plasma, using erythromycin as the internal standard (IS). A simple sample preparation method of protein precipitation with methanol was employed. Methanol, acetonitrile and water (12:30:58, v/v/v) were used as the isocratic mobile phase, with 0.1% formic acid and 0.1% ammonium acetate in water. Selected ion monitoring was specific for azithromycin and erythromycin. The assay was linear over the concentration range 4.69–600 ng/mL. The correlation coefficients for the calibration curves ranged from 0.9994 to 0.9998. The intra‐ and inter‐day precisions, calculated from quality control samples, were less than 8.24%. The method was employed in a pharmacokinetic study after oral administration of 500 mg azithromycin dispersible tablet to 20 healthy volunteers. Copyright © 2007 John Wiley & Sons, Ltd.