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Sensitive determination of bisphenol A and alkylphenols by high performance liquid chromatography with pre‐column derivatization with 2‐(4‐carboxyphenyl)‐5,6‐dimethylbenzimidazole
Author(s) -
Katayama Masatoki,
Sasaki Tadahiro,
Matsuda Yoshifumi,
Kaneko Satoru,
Iwamoto Teruaki,
Tanaka Masami
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.88
Subject(s) - chemistry , chromatography , derivatization , bisphenol a , phenol , calibration curve , detection limit , high performance liquid chromatography , methanol , fluorescence , organic chemistry , epoxy , physics , quantum mechanics
A new and sensitive high‐performance chromatographic method for the determination of bisphenol A and 8 alkylphenols with fluorescence detection is reported. Each phenol was derivatized by reaction with 2‐(4‐carboxyphenyl)‐5,6‐dimethylbenzimidazole at 40°C for 60 min. The fluorescence derivatives were separated on a Wakosil 5C18 column (4.0 i.d. × 300 mm, 5 µm) with methanol:water (10:90) as mobile phase (detection wavelength: λ ex 336 nm, λ em 440 nm). The detection limits were in the range of 0.1–10.0 pg/mL in serum. The calibration graphs were linear to 1.0 µg/mL. The relative standard deviations were 7.2–8.9%, respectively. The proposed method was applied to the determination of bisphenol A in mother and infant rat serum. Copyright © 2001 John Wiley & Sons, Ltd.Abbreviations used : CDB 2‐(4‐carboxyphenyl)‐5,6‐dimethylbenzimidazoleED endocrine discriptorIDC 1‐isopropyl‐3‐(3‐dimethylaminopropyl) carbodiimide perchlorate.