Premium
Determination and pharmacokinetic comparison of rhein in rats after oral dosed with Da‐Cheng‐Qi decoction and Xiao‐Cheng‐Qi decoction
Author(s) -
Tang WenFu,
Huang Xi,
Yu Qin,
Qin Feng,
Wan MeiHua,
Wang Yonggang,
Liang MaoZhi
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.873
Subject(s) - decoction , pharmacokinetics , chemistry , high performance liquid chromatography , chromatography , traditional medicine , bioavailability , pharmacology , medicine
Da‐Cheng‐Qi decoction (DCQD) is composed of dahuang, houpu, zhishi and mangxiao, while Xiao‐Cheng‐Qi decoction (XCQD) just contains dahuang, houpu, and zhishi. A validated high‐performance liquid chromatographic (HPLC) method was developed for the determination and pharmacokinetic comparison of rhein in rats. It was performed on a reversed‐phase C 18 column (150 × 4.6 mm) with a mobile phase of methanol and 0.2% acetic acid (89:11, v/v) and ultraviolet detection at 254 nm. The assay was linear over the range 30–3000 ng/mL ( r 2 = 0.9991), with mean percentage recovery of 101.4%. The RSD of intra‐ and inter‐day precision were less than 3.0%, with accuracy in the range 96.8–102.5% for each of the concentrations tested. Following oral administration, rhein plasma levels revealed curves characterized by peaks at 20.5 and 30.1 min corresponding to dosages of 15 mg/kg (XCQD) and 20 mg/kg (DCQD) at mean concentrations of 1184.67 and 1814.27 ng/mL, respectively. The half‐lives of rhein from XCQD and DCQD were 45.3 and 139.6 min, respectively. AUC (0–12h) of rhein after oral dosage with XCQD and DCQD were 3435.68 and 5382.25 µg/L h, respectively. The pharmacokinetic parameters may be used to guide the clinical prescription compatibility of traditional Chinese prescriptions related to dahuang and mangxiao. Copyright © 2007 John Wiley & Sons, Ltd.