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Simultaneous determination and pharmacokinetic study of water‐soluble and lipid‐soluble components of danshen in rat plasma using HPLC‐UV method
Author(s) -
Wang Xiuli,
Zhang ZhiRong,
Fu Hualin,
Liu Jie,
Chen Qian,
Nie Yu,
Deng Li,
Gong Tao
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.872
Subject(s) - chemistry , chromatography , phosphoric acid , high performance liquid chromatography , detection limit , pharmacokinetics , calibration curve , salvia miltiorrhiza , protocatechuic acid , pharmacology , antioxidant , biochemistry , medicine , alternative medicine , organic chemistry , traditional chinese medicine , pathology
A sensitive and specific HPLC‐UV method was developed for the simultaneous determination of major active components of danshen in rat plasma. Both water‐soluble and lipid‐soluble compounds were included, i.e. danshensu, salvianolic acid B and tanshinone IIA. Protocatechuic aldehyde and diazepam were used as internal standards. The chromatographic separation was achieved on a reversed‐phase C 18 column by gradient elution using acetonitrile and 0.025% (v/v) phosphoric acid solution as mobile phase, at a flow rate of 1.0 mL/min. Salvianolic acid B, danshensu and internal standards were detected at 281 nm, while the detection of tanshinone IIA was carried out at 272 nm. All calibration curves showed good linearity ( r 2 > 0.999) within test ranges. The limit of detection and the limit of quantification for danshensu, salvianolic acid B and tanshinone IIA in plasma were 0.065, 0.043, 0.022, 0.131, 0.085 and 0.044 µg/mL, respectively. This is the first report on the determination and pharmacokinetic study of danshensu, salvianolic acid B and tanshinone IIA in rat plasma and the results indicated that this method was reliable for the determination of the major active components of danshen in rat plasma. Copyright © 2007 John Wiley & Sons, Ltd.