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Determination of sodium tanshinone IIA sulfonate in plasma by liquid chromatography‐electrospray ionisation‐tandem mass spectrometry
Author(s) -
Hao Haiping,
Wang Guangji,
Cui Nan,
Li Jing,
Ding Zuoqi
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.871
Subject(s) - chemistry , chromatography , electrospray ionization , extraction (chemistry) , pharmacokinetics , mass spectrometry , liquid chromatography–mass spectrometry , tandem mass spectrometry , ammonium acetate , selected reaction monitoring , high performance liquid chromatography , medicine
Sodium tanshinone IIA sulfonate (STS) is a water‐soluble derivative of tanshinone IIa, an important lipophilic component contained in Salvia miltiorrhizae . A simple, sensitive and robust quantification method for STS based on LC‐ESI‐MS/MS was developed and validated, and has been successfully applied to the pharmacokinetic study. Liquid–liquid extraction was used for extracting STS from biological samples, with a satisfactory recovery exceeding 75% at all test concentrations. Isocratic mobile phase consisted of 75% acetonitrile and 25% water containing 0.005% ammonia acetate (pH 3). Good retention and baseline separation for STS and the selected internal standard, diclofenac sodium, were obtained on a Shim‐pack VP‐ODS analytical column under this condition. The method was linear in the concentration range of 1–500 ng/mL. The intra‐ and inter‐day precisions (RSD%) were within 9.0%. The deviation of the assay accuracies was within ±10.0%. STS was proved to be stable during all sample storing, preparation and analytic procedures. With a lower limit of quantitation at 1 ng/mL, this method has been proved to be sensitive enough for the pharmacokinetic study of STS. The plasma profile of STS followed a single intravenous dosing was well fitted to a three compartmental model. Copyright © 2007 John Wiley & Sons, Ltd.