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Separation and determination of psoralen and isopsoralen by microemulsion electrokinetic chromatography
Author(s) -
Zhang Huige,
Zhu Jinhua,
Qi Shengda,
Chen Xingguo,
Hu Zhide
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.859
Subject(s) - chemistry , psoralen , chromatography , analyte , detection limit , sodium dodecyl sulfate , extraction (chemistry) , microemulsion , micellar electrokinetic chromatography , electrokinetic phenomena , pulmonary surfactant , dna , biochemistry
The use of microemulsion electrokinetic chromatography was proposed to separate psoralen and isopsoralen in Psoralea corylitolia L. and its preparations. After conducting a series of optimizations, baseline separation was obtained for the analytes under the optimum conditions [sodium dodecyl sulfate 1.05% (m/v), ethyl acetate 0.96% (v/v), butan‐1‐ol 0.24% (v/v), 25 m m borate, pH 8.5, applied voltage 17.5 kV and detection at 254 nm]. Regression equations revealed linear relationships (correlation coefficients 0.9997 for psoralen and 0.9999 for isopsoralen) between the peak area of each analyte and the concentration. The limits of detection (defined as a signal‐to‐noise ratio of about 3) were 0.42 µg/mL for psoralen and 0.32 µg/mL for isopsoralen, respectively. The analytes were successfully determined with recoveries ranging from 95.50 to 102.03%. The method has been successfully applied for the analysis of psoralen and isopsoralen in medical samples. Furthermore, a simple and effective extraction method, with methanol in an ultrasonic water bath for 20 min three times, was used for sample preparation. Copyright © 2007 John Wiley & Sons, Ltd.