Premium
Precolumn fluorescence labeling method for simultaneous determination of hydroxyzine and cetirizine in human serum
Author(s) -
Hammad Sherin Farouk,
Mabrouk Mokhtar Mohamed,
Habib Ahmed,
Elfatatry Hamed,
Kishikawa Naoya,
Nakashima Kenichiro,
Kuroda Naotaka
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.848
Subject(s) - chemistry , chromatography , hydroxyzine , cetirizine , acetic acid , detection limit , triethylamine , metabolite , active metabolite , acetonitrile , solid phase extraction , fluorescence , pharmacology , organic chemistry , biochemistry , medicine , physics , quantum mechanics
Abstract A highly selective and sensitive method was developed for simultaneous determination of the antihistaminic drug hydroxyzine (HZ) and its pharmacologically active metabolite cetirizine (CZ) in human serum using haloperidol as internal standard. The method was based on fluorescence labeling of both drugs with a fluorescent arylboronic acid 4‐(4,5‐diphenyl‐1 H ‐imidazol‐2‐yl)phenyl boronic acid followed by separation on silica column using a mobile phase consisting of acetonitrile and water (90:10, v/v%) containing triethylamine and acetic acid. The labeling reaction conditions were optimized and the liquid–liquid extraction method was successfully applied to extract the both drugs from serum. The linearity range was 0.025–2.00 µg/mL for HZ and CZ. The limit of detection (S/N = 3) was 10 and 5 ng/mL for HZ and CZ, respectively. Copyright © 2007 John Wiley & Sons, Ltd.