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Development and evaluation of a rapid lateral flow immunochromatographic strip assay for screening 19‐nortestosterone
Author(s) -
Liu Liqiang,
Peng Chifang,
Jin Zhengyu,
Xu Chuanlai
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.832
Subject(s) - nitrocellulose , chemistry , chromatography , conjugate , polyclonal antibodies , reagent , colloidal gold , membrane , antibody , biochemistry , nanotechnology , immunology , nanoparticle , mathematical analysis , materials science , mathematics , biology
The lateral flow strip test for 19‐nortestosterone is one kind of immunochromatographic assay. Nitrocellulose membrane was separately immobilized with goat anti‐rabbit IgG (control line) and 19‐NT‐OVA conjugate (test line). Anti‐19‐NT polyclonal antibody labeled with colloidal gold particles acted as the detector reagent. The assay is qualitatively, not quantitatively, judged with positive or negative result. We tested the sensitivity of the strip using spiked swine urine, and each specimen was independently measured by LC/MS/MS. The sensitivity, measure by eye, was determined to be 200 ng/mL. The assay time was less than 15 min, and so suitable for on‐site rapid test. Copyright © 2007 John Wiley & Sons, Ltd.