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Determination and pharmacokinetics of manidipine in human plasma by HPLC/ESIMS
Author(s) -
Jing Jing,
Ren Weichao,
Chen Xijing,
He Hui,
Zhou Wei,
Zhu Xuping,
Sun Ya,
Wang Guangji
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.827
Subject(s) - chemistry , chromatography , pharmacokinetics , ammonium acetate , electrospray ionization , acetic acid , high performance liquid chromatography , detection limit , human plasma , metabolite , mass spectrometry , pharmacology , biochemistry , medicine
A sensitive HPLC/ESIMS method was established for the determination of manidipine in human plasma and pharmacokinetics study. After basified plasma with ammonia, manidipine and the internal standard (IS) (felodipine) were extracted with n ‐hexane and separated on a Hypersil ODS2 column with a mobile phase of methanol–5 m m ammonium acetate solution containing 0.1% acetic acid (85:15, v/v). MS determination was performed by electrospray ionization in the selected ion monitoring mode. Manidipine was monitored at m/z 611.4 and IS at m/z 384. The assay had a calibration range from 0.2 to 20 ng/mL and a lower limit of quantification of 0.1 ng/mL. The method has been successfully applied to the pharmacokinetic study in healthy volunteers. Copyright © 2007 John Wiley & Sons, Ltd.
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