Premium
Investigation of binding process of chlorpromazine to bovine serum albumin by means of passive and active experiments
Author(s) -
Rukhadze Marina D.,
Bezarashvili George S.,
Sidamonidze Nino Sh.,
Tsagareli Sonya K.
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.82
Subject(s) - chlorpromazine , chemistry , free fraction , bovine serum albumin , chromatography , albumin , fraction (chemistry) , serum albumin , plasma protein binding , biochemistry , endocrinology , medicine
The association process of chlorpromazine with bovine serum albumin by both onefactoral passive and multifactoral active experiments were investigated in model systems. The dependence of absolute concentration of the free fraction of chlorpromazine on the total concentration of chlorpromazine is described by the increasing function, but the dependence of the relative concentration (free/total) on this factor is influenced by external characteristics. The concentrations of chlorpromazine, bovine serum albumin and Tritons' mixture, as principal factors of a multifactoral experiment, influence the absolute and relative values of the free fraction of chlorpromazine non‐additively, ie the interaction of factors is significant. Copyright © 2001 John Wiley & Sons, Ltd.