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Comparison of HPLC and CE methods for the determination of cetirizine dihydrochloride in human plasma samples
Author(s) -
Kowalski Piotr,
Plenis Alina
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.818
Subject(s) - chromatography , cetirizine , chemistry , high performance liquid chromatography , derivatization , calibration curve , capillary electrophoresis , human plasma , extraction (chemistry) , detection limit , pharmacology , medicine
Two methods, capillary electrophoresis (CE) and high‐performance liquid chromatography (HPLC), for analysis of cetirizine dihydrochloride in small sample volumes of human plasma were compared. The CE and HPLC assays were developed and validated by analyzing a series of plasma samples containing cetirizine dihydrochloride in different concentrations using these two methods. The extraction procedure is simple and no complicated purification steps or derivatization are required. The analysis time in the HPLC method was shorter than that in the CE method, but solvent consumption was considerably lower in the CE method. The calibration curve was linear to at least 10–1000 ng/mL both for CE and HPLC with r 2 = 0.9993 and r 2 = 0.9994, respectively. The detection limits for cetirizine dihydrochloride were 3 and 5 ng/mL with CE and HPLC (a UV detector was applied in the both cases), respectively. Both methods were selective, robust and specific, allowing reliable quantification of cetirizine dihydrochloride, and could be useful for clinical and biomedical investigations. Copyright © 2007 John Wiley & Sons, Ltd.