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LC‐MS characterization of efficacy substances in serum of experimental animals treated with Sophora flavescens extracts
Author(s) -
Ye Guan,
Zhu HaiYan,
Li ZhiXiong,
Ma ChunHui,
Fan MingSong,
Sun ZhaoLin,
Huang ChengGang
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.805
Subject(s) - sophora flavescens , matrine , oxymatrine , chemistry , chromatography , electrospray ionization , sophora , traditional medicine , mass spectrometry , pharmacology , biology , medicine , traditional chinese medicine , alternative medicine , pathology
Anti‐DHBV (duck hepatitis B virus) activity was found in the aqueous extracts of Sophora flavescens Ait. in vivo . Liquid chromatography/electrospray ionization ion trap mass spectrometry was applied to characterize the components in duck serum after oral administration of S. flavescens extract. Oxymatrine ( 1 ), sophoranol ( 2 ), sophoridine ( 3 ) and matrine ( 4 ) were identified in the serum. Further research on the four compounds was evaluated for their antiviral activity against HBV (hepatitis B virus) in cell culture. The results suggested that oxymatrine, sophoranol and matrine were the efficacy substances for anti‐HBV activity in aqueous extracts of S. flavescens Ait. Copyright © 2007 John Wiley & Sons, Ltd.