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Quantitative LC/MS/MS method and pharmacokinetic studies of columbin, an anti‐inflammation furanoditerpen isolated from Radix Tinosporae
Author(s) -
Shi Qirong,
Liang Mingjin,
Zhang Weidong,
Zhang Chuan,
Liu Runhui,
Shen Yunheng,
Li Huiliang,
Wang Xiaolin,
Wang Xiangwei,
Pan Qiongqun,
Chen Chunlin
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.802
Subject(s) - chemistry , chromatography , pharmacokinetics , bioavailability , in vivo , oral administration , absorption (acoustics) , radix (gastropod) , liquid chromatography–mass spectrometry , methanol , tandem mass spectrometry , mass spectrometry , pharmacology , organic chemistry , medicine , physics , microbiology and biotechnology , acoustics , biology , botany
Columbin is an important component isolated from Radix Tinosporae . It has been demonstrated to possess many pharmacological activities, including anti‐inflammation, antitumor and inhibition of enzyme activity in vivo . The purpose of the present study was to examine in vivo pharmacokinetics and bioavailability of columbin in rats using a high‐performance liquid chromatography coupled with tandem mass spectrometry quantitative detection method. The columbin was extracted from rat plasma samples by methyl tert‐butyl ether, evaporated and reconstituted in 100 µL methanol prior to analysis. The separation was performed using a Luna reversed‐phase analytical column (5 µm, 100 × 2.0 mm) and an SB‐C 18 guard column (5 µm, 20 × 4.0 mm). The mobile phase was a mixture of methanol and water containing 25 mmoL/L NH 4 Ac (80:20, v/v). The method was validated within the concentration range of 5–5000 ng/mL, and the calibration curves were linear with correlation coefficients ( r ) >0.999. It was further applied to assess pharmacokinetics and oral bioavailability of columbin after i.v. and oral administration to rats. The oral bioavailability of columbin was only 3.18%, which indicated that columbin had poor absorption or underwent extensive first‐pass metabolism. Copyright © 2007 John Wiley & Sons, Ltd.