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Simultaneous quantitative and qualitative analysis of bioactive phenols in Dendrobium aurantiacum var. denneanum by high‐performance liquid chromatography coupled with mass spectrometry and diode array detection
Author(s) -
Yang Li,
Wang Yun,
Zhang Guangg,
Zhang Fang,
Zhang Zijia,
Wang Zhengtao,
Xu Luoshan
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.801
Subject(s) - chemistry , chromatography , formic acid , phenols , mass spectrometry , high performance liquid chromatography , solid phase extraction , detection limit , extraction (chemistry) , elution , quantitative analysis (chemistry) , analyte , sample preparation , analytical chemistry (journal) , organic chemistry
Abstract A novel high‐performance liquid chromatographic method with mass spectrometry and diode array detection method for the simultaneous qualitative and quantitative analysis of bioactive phenols was developed. In total, nine chemically diverse phenols including five bibenzyls, three phenanthrenes and a coumarin were unambiguously identified in Dendrobium aurantiacum var. denneanum by comparison with the available references or reported data according to their retention behaviors, UV spectra and fragmentations of ESI‐MS. The contents of the four main phenolic compounds, moscatilin, gigantol, moscatin and coumarin, in D. aurantiacum var. denneanum from the wild and various cultivated populations were determined by HPLC‐UV. The sample preparation involved a rapid and simple procedure based on solid‐phase extraction using a C 18 reversed‐phase cartridge. The quantitative analysis was performed on a Beckman Coulter™ ODS column (5 µm, 250 × 4.6 mm) using a linear gradient elution system of acetonitrile–0.5% formic acid. The method was validated for linearity, limits of detection (LOD) and quantification (LOQ), precision and accuracy. Good results were obtained with respect to the overall intra‐ and inter‐day variations (RSD less than 3.22%) and the percentage recoveries (ranging from 90.50 to 99.22%). Notable differences in the contents of phenols were observed among different cultivated populations. The samples colleted in April and May (spring), or October and November (autumn) accumulated much higher contents of phenols than those collected in other seasons. Copyright © 2007 John Wiley & Sons, Ltd.