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Liquid chromatographic–electrospray tandem mass spectrometric determination of bisoprolol in human plasma
Author(s) -
Liu Mingyuan,
Zhang Dan,
Sun Yantong,
Wang Yingwu,
Liu Zeyuan,
Gu Jingkai
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.785
Subject(s) - chemistry , chromatography , protein precipitation , formic acid , selected reaction monitoring , bisoprolol , tandem mass spectrometry , electrospray , ammonium acetate , liquid chromatography–mass spectrometry , pharmacokinetics , mass spectrometry , analyte , detection limit , high performance liquid chromatography , medicine , heart failure
An analytical method for the determination of bisoprolol in human plasma has been developed based on liquid chromatography–tandem mass spectrometry (LC–MS/MS). The analyte and internal standard (IS) diphenhydramine were cleaned up by protein precipitation with acetonitrile, reconstituted in mobile phase and separated by reversed‐phase high‐performance liquid chromatography (HPLC) using methanol:10 m m ammonium acetate:formic acid (70:30:0.1 v/v/v) as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) on an LC–MS/MS system and was completed within 2.5 min. The assay was linear over the range 0.5–100 ng/mL with a limit of quantitation (LOQ) of 0.5 ng/mL. The intra‐ and inter‐day precision levels were within 5.54 and 9.95%, respectively, while the accuracy was in the range 89.4–113%. This method has been utilized in a pharmacokinetic study, where healthy volunteers were treated with an oral dose of 5 mg bisoprolol. Copyright © 2007 John Wiley & Sons, Ltd.