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An HPLC method for the determination of ethacridine lactate in human urine
Author(s) -
Guo ZhiYong,
Wei DanYi,
Wang YuanYuan,
Xuan KunFei,
Yu XuFei,
Yu QiuLuan,
Chu Yun
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.780
Subject(s) - chemistry , chromatography , urine , calibration curve , detection limit , high performance liquid chromatography , extraction (chemistry) , methanol , coefficient of variation , linear range , correlation coefficient , biochemistry , statistics , mathematics , organic chemistry
An HPLC method was developed and validated for the determination of ethacridine lactate in human urine. Solid‐phase extraction cartridges were used to extract urine samples. Separation was carried out on a C 18 column maintained at 30°C with methanol–0.05% sodium dodecylsulfonate (70:30, v/v, pH 3) as mobile phase at a flow rate of 1.0 mL/min. Detection was at UV 272 nm. The calibration curve was linear in the concentration range of 4–4000 ng/mL, with linear correlation coefficient r equal to 0.9998. The limit of detection for the assay was 1.1 ng/mL. The within‐day accuracy ranged from 94.8 to 101.6% and precision from 2.3 to 5.4%. The between‐day accuracy ranged from 96.8 to 102.6% and precision from 4.0 to 5.3%. The absolute recovery was 95.4–101.2%. Urine samples were stable for at least 15 days if stored in the dark at −20°C. This simple and accurate method allows the sensitive determination of ethacridine lactate in human urine. It was successfully applied to assess the urine level of ethacridine lactate in women received intra‐amniotic injection. Copyright © 2007 John Wiley & Sons, Ltd.