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A high‐performance liquid chromatographic method for saikosaponin a quantification in rat plasma
Author(s) -
Tang Yihong,
Zhang Yuanyuan,
Zhu Haiyan,
Huang Chenggang
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.773
Subject(s) - chromatography , chemistry , detection limit , chromatographic separation , extraction (chemistry) , high performance liquid chromatography , pharmacokinetics , standard curve , quantitative analysis (chemistry) , linear range , pharmacology , medicine
A high‐performance liquid chromatographic method with UV detection has been developed for the determination of saikosaponin a in rat plasma. Saikosaponin a and internal standard jujuboside A were isolated from plasma samples by solid‐phase extraction. The chromatographic separation was achieved on a reversed‐phase C 18 column with the mobile phase of acetonitrile–water (35:65, v/v) at a flow rate of 1 mL/min and UV detection was set at 205 nm. The standard curve for saikosaponin a was linear over the concentration range 0.25–10 µg/mL and the limit of detection was 0.05 µg/mL. The absolute recovery was greater than 82%. The precision and accuracy ranged from 3.05 to 9.59% and 95.61 to 110.00%, respectively. The validated method was used to determine saikosaponin a in plasma samples in a pharmacokinetic study of saikosaponin a administered to Sprague–Dawley rats. Copyright © 2007 John Wiley & Sons, Ltd.