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Determination of hydroxysafflor yellow A in rat plasma and tissues by high‐performance liquid chromatography after oral administration of safflower extract or safflor yellow
Author(s) -
Li Yi,
Zhang Zhaoyang,
Zhang Jinlan
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.769
Subject(s) - chemistry , chromatography , protein precipitation , high performance liquid chromatography , acetic acid , calibration curve , methanol , oral administration , quantitative analysis (chemistry) , elution , gradient elution , detection limit , biochemistry , pharmacology , medicine , organic chemistry
A simple and reproducible HPLC method for quantification of hydroxysafflor yellow A (HSYA) in rat plasma and tissues after oral administration of safflower extract or safflor yellow (SY) was developed. Sample preparation was achieved by protein precipitation of plasma and tissue homogenates with three volumes of methanol. p ‐Hydroxybenzaldehyde was used as the internal standard (IS). HSYA and IS were separated on a Hypersil BDS‐C 18 column with a gradient elution system composed of acetonitrile and aqueous acetic acid. UV detection was used at 320 nm. The calibration curves were linear in all matrices ( r 2 > 0.999) in the concentration ranges 0.51–101.36 µg/mL for plasma, 12.27–2454.46 µg/g for intestines and 0.96–192.20 µg/g for lung. The intra‐day and inter‐day precision were all less than 12.5%, and the extract recovery was in the range 64.1–103.7% with RSD less than 15.6% for HSYA in all matrices. The method was used successfully to quantify HSYA in rat plasma and tissue samples to support a pharmacokinectic study. Copyright © 2007 John Wiley & Sons, Ltd.