Development and validation of an RP‐HPLC‐UV method for the determination of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug, in rat plasma | Zendy

Maltese Adriana | Zendy; Maugeri Francesco | Zendy; Ward Keith W. | Zendy; Bucolo Claudio | Zendy

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Development and validation of an RP‐HPLC‐UV method for the determination of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug, in rat plasma

Author(s) -

Maltese Adriana,

Maugeri Francesco,

Ward Keith W.,

Bucolo Claudio

Publication year - 2007

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.757

Subject(s) - chemistry , chromatography , triethylamine , coumarin , high performance liquid chromatography , acetonitrile , extraction (chemistry) , pharmacokinetics , chromatographic separation , linear range , drug , chromatography detector , detection limit , pharmacology , organic chemistry , medicine

A simple and rapid high‐performance liquid chromatographic method was developed and validated for the analysis in rat plasma of BOL‐303225‐A, a new coumarin‐based anti‐inflammatory drug. Liquid–liquid extraction was used for sample preparation. Chromatographic separation was achieved on a C 18 column using acetonitrile and water containing 1% triethylamine pH 3.5, adjusted with orthophosphoric acid (35.5:64.5 v/v) as mobile phase. The UV detector was set at 324 nm. The method proved to be linear ( r 2 > 0.99) and precise (RSD < 7%) over the concentration range 29–940 ng/mL, and was suitable for the support of pharmacokinetic studies in rats. Copyright © 2007 John Wiley & Sons, Ltd.

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