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Determination of atractylenolide II in rat plasma by reversed‐phase high‐performance liquid chromatography
Author(s) -
Ge Jian,
Wang Youwei,
Lu Xiaocong,
Sun Xiaohong,
Gong Fujun
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.756
Subject(s) - chemistry , chromatography , high performance liquid chromatography , ethyl acetate , quantitative analysis (chemistry) , detection limit , reversed phase chromatography
A method for quantitative determination of atractylenolide II in rat plasma using reversed‐phase high‐performance liquid chromatography (RP‐HPLC) coupled with UV spectrometry was established. From a variety of compounds and solvents tested, atractylenolide III was selected as the internal standard (IS) and ethyl acetate was found to be the best solvent for extracting atractylenolide II from plasma samples. RP‐HPLC analysis of the extracts was performed on an analytical column (DIKMA ODS, 150 × 4.6 mm; i.d., 5 µm) equipped with a security guard pre‐column system. There was good linearity over the range 0.05–5.0 µg/mL ( r > 0.99). The recoveries were more than 90.0% in plasma, and the intra‐ and inter‐day coefficients of variation were less than 10.0% in all cases. The limit of detection (LOD) was 0.025 µg/mL and the lower limit of quantification (LLOQ) was 0.05 µg/mL. The RP‐HPLC method was applied to quantitate atractylenolide II in rat plasma within 24 h in a pharmacokinetics study where experimental rats received a single dose of atractylenolide II (60 mg/kg). Copyright © 2007 John Wiley & Sons, Ltd.