Premium
Quantification of pseudoephedrine in human plasma by LC‐MS/MS using mosapride as internal standard
Author(s) -
Nirogi Ramakrishna V. S.,
Kandikere Vishwottam N.,
Shukla Manoj,
Mudigonda Koteshwara,
Maurya Santosh,
Komarneni Prashanth
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.745
Subject(s) - chemistry , chromatography , pseudoephedrine , bioequivalence , mosapride , detection limit , selected reaction monitoring , extraction (chemistry) , analyte , human plasma , tandem mass spectrometry , standard curve , electrospray , mass spectrometry , bioavailability , solid phase extraction , sample preparation , ephedrine , medicine , pharmacology , bioinformatics , neuroscience , biology
Abstract A simple, sensitive and rapid high‐performance liquid chromatography/positive ion electrospray tandem mass spectrometry (LC‐MS/MS) method was developed and validated for the quantification of pseudoephedrine in human plasma using mosapride as internal standard. Following solid‐phase extraction, the analytes were separated using an isocratic mobile phase on a reverse‐phase column and analyzed by MS/MS in the multiple‐reaction monitoring mode using the respective [M + H] + ions, m/z 166/148 for pseuoephedrine and m/z 422/198 for the IS. The method exhibited a linear dynamic range of 2–1000 ng/mL pseudoephedrine in human plasma. The lower limit of quantification was 2 ng/mL with a relative standard deviation of less than 9% for pseudoephedrine. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The total chromatographic run time of 2 min for each sample made it possible to analyze more than 400 human plasma samples per day. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic, bioavailability or bioequivalence studies. Copyright © 2007 John Wiley & Sons, Ltd.