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Semi‐micro column high‐performance liquid chromatography with UV detection for quantification of aspirin and salicylic acid and its application to patients' sera administered with low‐dose enteric‐coated aspirin
Author(s) -
Ohwaki Yuichi,
Yamane Tomoko,
Ishimatsu Takashi,
Wada Mitsuhiro,
Nakashima Kenichiro
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.742
Subject(s) - chemistry , salicylic acid , chromatography , aspirin , phosphoric acid , metabolite , high performance liquid chromatography , detection limit , column chromatography , solid phase extraction , extraction (chemistry) , biochemistry , organic chemistry
A simultaneous determination of aspirin (ASA) and its metabolite, salicylic acid (SA), in human serum by a semi‐micro column HPLC‐UV was developed. A relatively small size of serum sample (100 µL) containing ASA and SA was cleaned up by a simple solid phase extraction. A good separation of ASA and SA could be achieved within 25 min using a semi‐micro ODS column with an eluent of MeOH/0.7 m m phosphoric acid solution (pH 2.5) = 50:50 (v/v). The calibration curves for ASA and SA showed good linearity ( r = 0.999) with the detection limits 114 and 38 ng/mL at a signal‐to‐noise ratio of 3, respectively. ASA and SA in patients' sera administered with low‐dose enteric‐coated aspirin were determined, and the concentration ranges obtained for ASA and SA were 1.2–2.2 and 0.5–57.3 µg/mL, respectively. Copyright © 2007 John Wiley & Sons, Ltd.