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A solid‐phase extraction method for high‐performance liquid chromatographic determination of salvianolic acid B in rabbit plasma: application to pharmacokinetic study
Author(s) -
Ma Yueming,
Wang Tianming
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.741
Subject(s) - chemistry , chromatography , trifluoroacetic acid , high performance liquid chromatography , calibration curve , pharmacokinetics , extraction (chemistry) , analyte , methanol , acetonitrile , detection limit , pharmacology , organic chemistry , medicine
A sensitive solid‐phase extraction/high‐performance liquid chromatographic method with ultraviolet detection was established for the analysis of salvianolic acid B in rabbit plasma. The analyte was separated on a reversed‐phase column with trifluoroacetic acid–methanol–acetonitrile (70:10:20, v/v/v) as mobile phase at a flow rate of 1 mL/min, and ultraviolet detection at 315 nm. The calibration curve for salvianolic acid B was linear over the range 35–1400 µg/L with coefficients of correlation >0.999. The inter‐day and intra‐day precisions of analysis were <15%, and assay accuracy ranged from 95.3 to 109.1%. This method is suitable for determining salvianolic acid B in plasma and thus investigating the pharmacokinetics of salvianolic acid B. Copyright © 2007 John Wiley & Sons, Ltd.