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Post TLC developing technique for tyrosinase inhibitor detection
Author(s) -
Wangthong Sakulna,
Tonsiripakdee Ilhum,
Monhaphol Thitinun,
thabenjawan Ratchanee,
Wanichwecharungruang Supason Pattanaargson
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.727
Subject(s) - tyrosinase , chemistry , chromatography , biochemistry , enzyme
Post TLC developing technique was developed to detect substances which can inhibit tyrosinase activity. The method involved spraying the TLC plate or chromatographic paper containing sample spot(s) with tyrosinase and l ‐tyrosine solutions successively. A positive result could be visualized directly as white spot(s) against a brownish‐purple background. The method can either be used as a quick screening method for tyrosinase inhibitor detection or a guiding procedure for an isolation of tyrosinase inhibitors from mixtures or natural product extracts. The technique is sensitive enough to give a clear result in the presence of only 6 ng glabridin. Copyright © 2006 John Wiley & Sons, Ltd.