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High‐performance liquid chromatography and LC‐ESI‐MS method for the identification and quantification of two biologically active polyisoprenylated benzophenones xanthochymol and isoxanthochymol in different parts of Garcinia indica
Author(s) -
Kumar Satyanshu,
Chattopadhyay Sunil K.
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.726
Subject(s) - chemistry , garcinia , chromatography , electrospray ionization , high performance liquid chromatography , resolution (logic) , mass spectrometry , electrospray , traditional medicine , medicine , artificial intelligence , computer science
Abstract A reverse‐phase high‐performance liquid chromatographic method was developed for qualitative and quantitative analysis of xanthochymol ( 1 ), and isoxanthochymol ( 2 ) in the fruit rinds, leaves and seed pericarps of Garcinia indica with confirmation using PDA detection and electrospray ionization MS. Absorption at 276 nm was chosen as the measuring wavelength at which resolution and baseline separation of compounds ( 1 ) and ( 2 ) could be obtained. The identity of the above two isomeric compounds ( 1 ) and ( 2 ) in the samples was unambiguously determined by their respective quasi‐molecular ion [M – H] − in ESI‐MS. Compounds ( 1 ) and ( 2 ) were qualitatively and quantitatively analyzed in the above three samples of Garcinia indica . The overall analytical procedure is rapid and reproducible and is considered for the analysis of the above two compounds. Copyright © 2006 John Wiley & Sons, Ltd.