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Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography–tandem mass spectrometry
Author(s) -
Ding CunGang,
Zhou Zhen,
Ge QingHua,
Zhi XiaoJin,
Ma LiLi
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.723
Subject(s) - chemistry , chromatography , glipizide , electrospray ionization , tandem mass spectrometry , selected reaction monitoring , gliclazide , mass spectrometry , liquid chromatography–mass spectrometry , pharmacokinetics , phenformin , metformin , diabetes mellitus , pharmacology , medicine , endocrinology
A method has been developed for the simultaneous quantification of metformin (I) and glipizide (II) in human plasma. It is based on high‐performance liquid chromatography with electrospray ionization tandem mass (LC‐ESI‐MS/MS) spectrometric detection in positive ionization mode. Phenformin (III) and gliclazide (IV) were used as internal standards for I and II, respectively. The MS/MS detection was performed in multiple reaction monitoring (MRM) mode. The precursor‐product ion combinations of m / z 130 → 71, 446 → 321, 206 → 60 and 324 → 127 were used to quantify I, II, III and IV, respectively. This method was validated in the concentration ranges of 0.02–4 µg/mL for I and 0.004–0.8 µg/mL for II. It was utilized to support a clinical pharmacokinetic study after single dose oral administration of a combination of I and II. Copyright © 2007 John Wiley & Sons, Ltd.