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A rapid liquid chromatography–tandem mass spectrometry method for quantification of a biologically active molecule camboginol in the extract of Garcinia cambogia
Author(s) -
Chattopadhyay Sunil K.,
Kumar Satyanshu
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.719
Subject(s) - chemistry , chromatography , selected reaction monitoring , calibration curve , tandem mass spectrometry , mass spectrometry , liquid chromatography–mass spectrometry , acetic acid , garcinia , acetonitrile , reproducibility , coefficient of variation , quantitative analysis (chemistry) , detection limit , organic chemistry , medicine , traditional medicine
A liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the determination of camboginol in the extract of fruit rinds of Garcinia cambogia has been developed. Separation was achieved isocratically on an RP C 18 column using a solvent system consisting of a mixture of acetonitrile–water (9:1) and methanol–acetic acid (99.5:0.5) in the ratio of 30:70 as mobile phase at a flow rate of 0.4 mL/min. A multiple reaction monitoring (MRM) method was developed for quantification of camboginol in the fruit rinds extract of G. cambogia using MRM transitions of m / z 601.4 → m / z 176.7 and m / z 601.4 → m / z 448.9, respectively. The calibration curve based on peak area against concentration was linear up to 50 ng/mL with a detection limit of 0.5 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 6%. The method was successfully applied for quantification of camboginol in different Garcinia extracts. Copyright © 2006 John Wiley & Sons, Ltd.