A rapid liquid chromatography–tandem mass spectrometry method for quantification of a biologically active molecule camboginol in the extract of Garcinia cambogia

A liquid chromatography–tandem mass spectrometry (LC‐MS/MS) method for the determination of camboginol in the extract of fruit rinds of Garcinia cambogia has been developed. Separation was achieved isocratically on an RP C 18 column using a solvent system consisting of a mixture of acetonitrile–water (9:1) and methanol–acetic acid (99.5:0.5) in the ratio of 30:70 as mobile phase at a flow rate of 0.4 mL/min. A multiple reaction monitoring (MRM) method was developed for quantification of camboginol in the fruit rinds extract of G. cambogia using MRM transitions of m / z 601.4 → m / z 176.7 and m / z 601.4 → m / z 448.9, respectively. The calibration curve based on peak area against concentration was linear up to 50 ng/mL with a detection limit of 0.5 ng/mL. The method showed satisfactory reproducibility with a coefficient of variation of less than 6%. The method was successfully applied for quantification of camboginol in different Garcinia extracts. Copyright © 2006 John Wiley & Sons, Ltd.