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Application of capillary electrophoresis in the analysis of novel synthetic dideoxynucleoside analogues with potential anti‐HIV activity
Author(s) -
Balayiannis G.,
Papaioannou D.,
Karamanos N. K.
Publication year - 2001
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.71
Subject(s) - chemistry , capillary electrophoresis , thymine , uracil , purine , pyrimidine , electrophoresis , nucleobase , chromatography , stereochemistry , dna , biochemistry , enzyme
The aim of this study was the development of a capillary electrophoretic method for the analysis of a series of novel synthetic dideoxynucleoside analogues with potential anti‐HIV activity. These analogues consist of a tetrahydrofuranyl or a tetrahydropyranyl ring as the pseudosugar part and bear a hydroxyethyl side‐chain and a nucleobase of the pyrimidine (eg thymine or uracil) or the purine (adenine) type with cis or trans configuration. Analysis of these derivatives was performed by capillary zone electrophoresis using 25 m M phosphate pH 3.00 and 4.00 as operating buffers for pyrimidine and purine analogues, respectively, and detection of separated species at 254 nm. Copyright © 2001 John Wiley & Sons, Ltd. Abbreviations used: ddN 2′,3′‐dideoxynucleosidesiso‐ddN isodideoxynucleosides.