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A liquid chromatography–mass spectrometry method for the quantitation of aristololactam‐I in rat plasma
Author(s) -
Ling Zhen,
Jiang Zhenzhou,
Huang Xin,
Zhang Luyong,
Xu Xiaoyue
Publication year - 2007
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.707
Subject(s) - chemistry , chromatography , mass spectrometry , electrospray ionization , acetic acid , electrospray , methanol , biochemistry , organic chemistry
A sensitive method with liquid chromatography–electrospray ionization mass spectrometry has been developed and validated for the determination of aristololactam‐I in rat plasma after oral administration of aristolochic acid‐I using finesteride as the internal standard. Chromatographic separation was achieved on a Lichrospher C 18 column using methanol:0.05% acetic acid in water (71:29, v/v) as a mobile phase delivered at a flow rate of 1 mL/min. The assay was linear for aristololactam‐I over the range 0.3–300 ng/mL. The analysis of quality control samples demonstrated precision with coefficient of variation less than 20% ( n = 5). Absolute recovery of aristololactam‐I was 90.4–97.3%. The LC‐MS method for the determination of aristololactam‐I is sensitive, specific and can be used to investigate the toxicokinetics of aristololactam‐I. Copyright © 2006 John Wiley & Sons, Ltd.