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Determination of coenzyme Q 10 in human breast milk by high‐performance liquid chromatography
Author(s) -
Tang Peter H.,
Miles Michael V.,
Steele Paul,
Davidson Barbara S.,
Geraghty Sheela R.,
Morrow Ardythe L.
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.702
Subject(s) - coenzyme q10 , chemistry , chromatography , high performance liquid chromatography , detection limit , breast milk , coenzyme q – cytochrome c reductase , extraction (chemistry) , chromatographic separation , biochemistry , cytochrome c , mitochondrion
An isocratic HPLC method was developed for the determination of coenzyme Q 10 (CoQ 10 ) in human breast milk. After a single‐step liquid–liquid extraction, the milk extract was injected directly into the HPLC system. The analytical method is based on pre‐column inline treatment of CoQ 10 . Chromatographic separation of CoQ 10 and coenzyme Q 9 (CoQ 9 ) internal standard was achieved using a reversed‐phase Microsorb‐MV C 18 analytical column. CoQ 10 and CoQ 9 were monitored by an electrochemical detector (ECD). An excellent linearity ( r = 0.999) was observed for CoQ 10 in the concentration range 0.06–2.5 µmol L −1 in breast milk. The limit of quantitation (LOQ) was 60 nmol L −1 . Coefficients of variations (CVs) for intra‐day and inter‐day assay precisions were less than 5%. A total of 194 breast milk samples were analyzed for the CoQ 10 concentration; the mean value was 0.32 ± 0.21 µmol L −1 . Copyright © 2006 John Wiley & Sons, Ltd.