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Determination and pharmacokinetic study of syringin and chlorogenic acid in rat plasma after administration of Aidi lyophilizer
Author(s) -
Li Qing,
Sun LiXin,
Xu Liang,
Jia Ying,
Wang ZhiWei,
Shen ZhenDuo,
Bi KaiShun
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.698
Subject(s) - chemistry , chlorogenic acid , pharmacokinetics , chromatography , pharmacology , plasma concentration , medicine
Abstract A high‐performance liquid chromatographic (HPLC) method was developed for the first time to simultaneously quantify syringin and chlorogenic acid in rat plasma using wavelength‐transfer technology. The analysis was performed on a Diamonsil C 18 column (200 × 4.6 mm i.d., 5 µm particle size) with isocratic mobile phase consisting of acetonitrile–0.05% phosphoric acid (12:88, v/v). The linear ranges were 0.20–10 and 0.25–30 µg/mL, respectively. The lower limits of quantification were 0.20 and 0.25 µg/mL, respectively. The method was shown to be reproducible and reliable with intraday precision below 8.5 and 6.1%, interday precision below 7.1 and 5.5%, accuracy within ±7.1 and ±8.6%, and mean extraction recovery excess of 92.1 and 80.9%, respectively, which were all calculated from the blank plasma sample spiked with syringin and chlorogenic acid at three concentrations of 0.20, 1.0 and 6.0 µg/mL for syringin and 0.25, 2.0 and 20 µg/mL for chlorogenic acid. This method was validated for specificity, accuracy and precision and was successfully applied to the pharmacokinetic study of syringin and chlorogenic acid in rat plasma after intravenous administration of Aidi lyophilizer. Copyright © 2006 John Wiley & Sons, Ltd.