Premium
Identification of volatile components in Angelica species using supercritical‐CO 2 fluid extraction and solid phase microextraction coupled to gas chromatography‐mass spectrometry
Author(s) -
Kim Mi Ra,
ElAty A. M. Abd,
Choi JeongHeui,
Lee Kang Bong,
Shim Jae Han
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.696
Subject(s) - chemistry , angelica sinensis , chromatography , solid phase microextraction , gas chromatography–mass spectrometry , mass spectrometry , gas chromatography , supercritical fluid , supercritical fluid extraction , rhizome , extraction (chemistry) , supercritical fluid chromatography , sample preparation , traditional medicine , traditional chinese medicine , organic chemistry , medicine , alternative medicine , pathology
As a part of our search for environmentally friendly solvents to extract the active components of medicinal plants, two sampling techniques, supercritical fluid extraction (SFE) using CO 2 and solid‐phase microextraction (SPME) were compared for their efficacy in the analysis of volatiles rhizome components emitted from the medicinal herbs Angelica gigas NAKAI (Korean danggui), Angelica sinensis (Chinese danggui), and Angelica acutiloba (Japanese danggui). A total of 54 compounds released from all of these varieties of Angelica rhizomes were separated and identified by gas chromatography–mass spectrometry (GC‐MS). The composition of supercritical extracts from these plants was very different from the solid‐phase microextraction products. More compounds were detected by SPME–GC–MS (41) than by SFE‐GC–MS (17). The results of these analyses suggest that SFE may be useful for detecting the main components, decursinol angelate and decursin in Korean danggui, and butylidene dihydro‐phthalide in both Chinese and Japanese danggui, whereas the results for SPME did not. The SFE method required specialized instrumentation, required little time to prepare the sample, and had a small sample size and no organic solvent. In sum, these results suggest that SFE is useful for extracting the volatile main components of danggui cultivars. Its simplicity, low cost and speed may allow SPME to increase the recovery of volatile components in general without disturbing the main components of the plant. Copyright © 2006 John Wiley & Sons, Ltd.