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High‐performance liquid chromatographic method for the determination of mangiferin in rat plasma and urine
Author(s) -
Wang Hui,
Ye Guan,
Tang YiHong,
Zhu HaiYan,
Ma RongRong,
Sun ZhaoLing,
Huang ChengGang
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.695
Subject(s) - mangiferin , chromatography , chemistry , urine , rutin , detection limit , high performance liquid chromatography , standard curve , acetic acid , biochemistry , antioxidant
A reversed‐phase high‐performance liquid chromatography assay for mangiferin in rat plasma and urine was developed. Rutin was employed as an internal standard. The mobile phase consisted of acetonitrile–water (16:84, v/v) containing 3% acetic acid at a flow rate of 1 mL/min. Detection was at 257 and 365 nm for mangiferin in plasma and urine, respectively. The limit of quantitation (LOQ) of mangiferin was 0.6 mg/mL in plasma, and 0.48 mg/mL in urine. The standard curve was linear from 0.6 to 24 mg/mL in plasma, and 0.48 to 24 mg/mL in urine, both intra‐ and inter‐day precision of the mangiferin were determined and their RSD did not exceed 10%. The method provides a technique for rapid analysis of mangiferin in rat plasma and urine, which can be used in pharmacokinetic studies. Copyright © 2006 John Wiley & Sons, Ltd.
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