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Application of microdialysis to study the in vivo release of pingyangmycin from in situ gels in rabbits
Author(s) -
Gao Zibin,
Ding Pingtian,
Zhang Li,
Yuan Zhenting,
Shi Jian,
Wei Jing,
Chen Dawei
Publication year - 2006
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.688
Subject(s) - microdialysis , chemistry , chromatography , in vivo , in situ , high performance liquid chromatography , detection limit , biochemistry , extracellular , microbiology and biotechnology , organic chemistry , biology
Microdialysis was used together with HPLC to monitor the local concentration of pingyangmycin hydrochloride (PYM) after embolizing rabbit ear‐veins with an injectable sustained release formulation, PYM‐Zein/PYM‐Zein‐sucrose acetate isobutyrate (SAIB), in situ gel. The dialysis probe was perfused at 2 µL/min. The in vivo recovery was 46.6 3.1% ( n = 4). The samples were injected directly into HPLC. PYM was detected using UV detector at 291 nm. Separation from other components in the dialysate was performed using a Discovery ® RP‐Amide C 16 column within 12 min. The response for PYM in the dialysate was linear ( r 2 > 0.996) over the range of 2.5–212 µg/mL. The limit of detection and limit of quantitation of PYM in the dialysate were 0.4 and 1.5 µg/mL, respectively. The results demonstrated that the in situ gel of PYM‐Zein‐SAIB could extend the release of PYM to 4 days. SAIB could significantly cut down the initial burst of PYM from the in situ gels ( p < 0.05). Copyright © 2006 John Wiley & Sons, Ltd.